Summary: The enzymatic addition of C-terminal azides to polypeptides (ENZ-A-CAP for short) aims to expand on Quantum-Si’s Platinum® single-molecule sequencing platform to enable protein sequencing of peptides generated with any digest patterns and hopes to extend this feature to full-length proteins for full proteoform sequencing.
Summary: Sequencing proteins and correlating amino acid changes to biological function is critical to advancing our understanding of human health and disease. Next-generation protein sequencing (NGPS) on Platinum® enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument. To demonstrate the versatility of Platinum and the […]
Summary: Antibodies are a paradigm for high-affinity, protein-based binding reagents and are extremely important in biotechnological, diagnostic, and therapeutic applications. Of special interest are nanobodies, recombinant variable domains from heavy-chain-only antibodies. Nanobodies have several advantages: their small molecular weight, superior solubility and stability and clearance rate. One particular use of nanobodies is their use in […]
Summary: Single-molecule methods provide useful insights into the intermolecular variations and functional differences of individual molecules and have revealed much about the complexity of biological processes. Today it is possible to monitor binding kinetics at the single molecule level in a highly parallel fashion using zero-mode waveguide (ZMW) arrays. This allows monitoring the binding/unbinding of […]
Summary: Next-gen protein sequencing is a transformational tool for protein science to unlock new insights into the function of proteins in health and diseases. Quantum-Si’s Platinum® technology brings the insights of protein sequencing to every lab with a space-friendly benchtop instrument, a simple end-to-end workflow, and single-molecule resolution that enables detection of protein variants and […]
Summary: Next-gen protein sequencing is a transformational tool for protein science to unlock new insights into the function of proteins in health and diseases. Quantum-Si’s Platinum technology brings the insights of protein sequencing to every lab with a space-friendly benchtop instrument, a simple end-to-end workflow, and single-molecule resolution that enables detection of protein variants and […]
Studies of the proteome would benefit greatly from methods to directly sequence and digitally quantify proteins and detect post-translational modifications with single-molecule sensitivity. Quantum-Si has developed the Platinum® next-generation protein sequencing platform to offer a convenient benchtop solution for direct sequencing of single protein molecules with the aim of making protein sequencing accessible to any […]
Next-gen protein sequencing is a transformational tool for protein science to unlock new insights into the function of proteins in health and diseases. Quantum-Si’s Platinum technology brings the insights of protein sequencing to every lab with a space-friendly benchtop instrument, a simple end-to-end workflow, and single-molecule resolution that enables detection of protein variants and modifications. […]
Aberrant post-translational modifications (PTMs) on arginine residues, namely methylation and citrullination, are closely linked to oncogenic processes. Detection of these PTMs is challenging with current technologies and can be hampered by low abundance and mass differences that are difficult to resolve by mass spectrometry. To overcome these hurdles, we developed methods for PTM detection using […]
Next-gen protein sequencing is a transformational tool for protein science to unlock new insights into the function of proteins in health and disease. Quantum-Si’s Platinum technology brings the insights of protein sequencing to every lab with a space-friendly benchtop instrument, a simple end-to-end workflow, and single-molecule resolution that enables detection of protein variants and modifications […]