Achieve Unparalleled Antibody Sequencing and Characterization with Platinum®

Browse our application notes to uncover the potential of using Platinum’s Next-Generation Protein Sequencing to characterize antibodies for advancing research.

• Enrichment and Sequencing of Albumin Protein from Urine – APP NOTE

  In this application note, we explore the enrichment and sequencing of albumin protein from urine using the Platinum next-generation protein sequencing™ workflow. Albumin is an important biomarker for disease diagnosis, and urine offers a non-invasive and easily accessible source for monitoring patient conditions. We describe a workflow that combines affinity-based enrichment with Quantum-Si’s protein sequencing technology to identify and map albumin peptides.
  Download the application note to learn more about this powerful method for analyzing biomarkers and protein variants in biofluids.

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  Here we illustrate the protein sequence of HSA after LysC digestion. These findings demonstrate the effectiveness of the Platinum workflow in accurately identifying and mapping albumin peptides.

  The traces for each HSA peptide identified by the software analysis pipeline represent the fluorescence signal recorded during sequencing. By examining these traces, we were able to gain insights into the sequencing data and verify the accuracy of the peptide identification.

  

• Immunoprecipitation of IL-6 from Human Serum for Next- Generation Protein Sequencing™ on Platinum® – APP NOTE

  The application note explores the enhancement of scarce proteins from complex biological matrices, a crucial phase in numerous proteomic workflows. It presents a cutting-edge immunoprecipitation method tailored for Quantum-Si’s advanced protein sequencing workflow. This on-bead digestion approach directly formulates digested peptide libraries, eliminating the need for a sample elution step. Its efficacy is demonstrated by enriching IL-6 from human serum and subsequently sequencing and identifying it with Platinum.

  See the transformative power of this novel immunoprecipitation method by accessing the complete application note now.

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  The application note showcases an all-inclusive process for on-bead digestion and immunoprecipitation of proteins, setting the stage for cutting-edge protein sequencing. This detailed procedure underscores the smooth progression from protein capture to its readiness for sophisticated sequencing, underscoring the method’s innovative and efficient nature.

  Here we highlight the nuanced data of traces, coverage, and pulse duration for peptides identified in IL-6 immunoprecipitated from PBS. Employing five recognizers, the research effectively pinpoints 12 amino acids, resulting in the detection of three unique peptides.

  

  

  This insightful analysis showcases the count of aligned IL-6 peptides found in each immunoprecipitated serum sample. Beyond highlighting the method’s efficacy, the data provides a deeper understanding of the correlation between input concentration and sequencing results.

• Identifying Monoclonal Antibodies with Quantum-Si’s Next- Generation Protein Sequencing™ Technology – APP NOTE

  Detecting rare monoclonal antibodies within a polyclonal antibody group is vital for grasping disease processes, diagnostics, among other aspects. While conventional techniques have their merits, they often entail significant expenses, intricacy, and lengthy protocols. This application note unveils Quantum-Si’s advanced protein sequencing approach using the Platinum device, offering a remedy to these hurdles and adeptly pinpointing these essential antibodies.

  Discover this revolutionary approach and its prospective significance by accessing the complete application note.

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  Here we highlight the sequencing data of distinct peptides originating from two separate monoclonal antibodies. Gathered over a span of 10 hours, the data illustrates the recognition segments (RS) with coverage denoted by the color intensity in each box, paired with the pulse duration distribution. This depiction aptly showcases the Platinum instrument’s proficiency in precise sequencing and pinpointing peptides exclusive to each antibody.