Empower a New Era of Protein Biomarker Development on Platinum® Pro
Uncover disease biomarkers with the precision of single-molecule protein sequencing.
Now you can interrogate disease biomarkers using our advanced next-gen protein sequencer.
Why choose Platinum Pro for biomarker development?
Characterize biomarkers identified in high-throughput proteomic screening assays
Reveal biomarkers that go undetected with conventional methods
Identify binding partners in enrichment techniques such as IP, co-IP, and chromatography
Enable multiomics research by seamlessly integrating proteomics with genomics and transcriptomics
Biomarker development is essential for drug development, early disease detection, personalized therapies, and gaining a deeper understanding of disease progression. When it comes to biomarker identification, choosing our platform offers researchers a comprehensive solution that unlocks deeper insights into the proteome.
Platinum Pro’s single-molecule protein sequencing technology allows for more detailed and accurate analysis of a wide range of proteins, revealing critical biomarkers that may go undetected with conventional approaches. Platinum Pro makes it easier to validate potential biomarkers and bring precision medicine closer to reality. With integrated informatics for simplifying complex data analysis, our platform provides a scalable solution that grows with your research, supporting both current projects and future advancements.
Explore our resources to discover how Platinum Pro can enhance your biomarker development:
The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, the team at Northwestern University evaluated the complementarity of two cutting-edge proteomic technologies—single-molecule protein sequencing and individual ion mass spectrometry—for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteoform levels. For single-molecule protein sequencing, they employed the Platinum® instrument. NGPS on Platinum utilizes cycles of N-terminal amino acid recognizer binding and aminopeptidase cleavage to enable parallelized sequencing of single peptide molecules. They found that NGPS produces single amino acid coverage of multiple key regions of IL-6, including two peptides within helices A and C which harbor residues that reportedly impact IL-6 function. For top-down proteoform evaluation, the team used individual ion mass spectrometry (I2MS), a highly parallelized orbitrap-based charge detection MS platform. Single ion detection of gas-phase fragmentation products (I2MS2) gives significant sequence coverage in key regions in IL-6, including two regions within helices B and D that are involved in IL-6 signaling. Together, these complementary technologies delivered a combined 52% sequence coverage, offering a more complete view of IL-6 structural and functional diversity than either technology alone.
Northwestern University’s Neil Kelleher and his team have a published article, featured in Springer’s Analytical and Bioanalytical Chemistry journal. The paper highlights the synergy of complementary protein detection methods to more comprehensively cover protein segments relevant to biological interactions.
Protein variants of the same gene–proteoforms–can have high molecular similarity yet exhibit different biological functions. Thus, identifying unique peptides that unambiguously map to proteoforms can provide crucial biological insights. In humans, four human tropomyosin (TPM) genes produce similar proteoforms that can be challenging to distinguish with standard proteomics tools.
Dr. Gloria Sheynkman and her team at the University of Virginia School of Medicine have published a paper in the Journal of Proteome Research showcasing the capabilities of the Platinum next-gen protein sequencer in advancing their research in this complex area of study.
The application note delves into the enrichment of low-abundant proteins from intricate biological matrices, a pivotal step in many proteomic workflows. It introduces an innovative immunoprecipitation protocol that aligns with Quantum-Si’s next-gen protein sequencing workflow. By bypassing the sample elution step, this on-bead digestion protocol directly prepares digested peptide libraries. The effectiveness of this method is showcased through the enrichment of IL-6 from human serum, followed by its sequencing and identification using Platinum.
Understand the potential of this groundbreaking immunoprecipitation protocol by downloading the full application note today.
The application note highlights a comprehensive workflow for on-bead digestion and immunoprecipitation of proteins, paving the way for next-generation protein sequencing. This step-by-step process emphasizes the seamless transition from protein capture to its preparation for advanced sequencing, highlighting the efficiency and innovation of the approach.
Here we showcase the intricate details of the traces, coverage, and pulse duration data for the peptides identified in IL-6 immunoprecipitated from PBS. Utilizing five recognizers, the study successfully identifies 12 amino acids, leading to the recognition of three distinct peptides.
This compelling analysis presents the number of aligned IL-6 peptides discovered in each immunoprecipitated serum sample. This data not only underscores the efficiency of the method but also offers insights into the relationship between input concentration and sequencing outcomes.