Unlock the Power of Next-Generation Protein Sequencing
New proteomic discoveries are now possible without the need for complex workflows and bioinformatics expertise. Confidently correlate biological function with changes in protein sequence and post-translational modifications.
See Beyond the Genome with Quantum-Si
While next-generation DNA sequencing greatly advanced our understanding of biology and what genes could influence biological function, protein sequencing is necessary to capture what is actually happening in the body now.
With the Platinum™ single-molecule protein sequencing platform, you can obtain this critical information quickly, efficiently, and from the convenience of your own lab. With Platinum, protein sequencing is:
- Convenient—the benchtop instrument and collaboration-friendly cloud-based analysis software easily fit into everyone’s lab and research projects.
- Accessible—with simple, straightforward workflows and automated cloud-based analysis software, any lab can generate protein sequence and PTM data.
- Insight-generating—single amino acid variation and PTMs can be detected directly by sequencing without the need to acquire or generate highly specific affinity reagents directed against these variants and modifications.
How It Works
Prepare
Proteins are digested with a protease and individual peptides are immobilized into wells on our semiconductor chip followed by the addition of fluorescently-labeled N-terminal amino acid (NAA) recognizers and aminopeptidases.
Sequence
Recognizers bind NAAs of the immobilized peptides and the fluorescence signal from these binding events is recorded by the chip. The fluorescence signal, including fluorescence lifetime and intensity, is unique for each recognizer. Repeated binding events result in the collection of high-precision kinetic data that can discriminate single amino acids and PTMs and permit a single recognizer to accurately detect more than one type of NAA. The aminopeptidases sequentially cleave one NAA at a time from the peptides over the duration of the sequencing run, exposing the next amino acid for recognition in a process that continues until the entire peptide is sequenced.
Analyze
Data is processed in our automated, cloud-based system. Protein sequencing data can be interpreted instantly without the need for bioinformatics expertise.
How Quantum-Si’s Technology Differs from Edman Degradation
Quantum-Si’s single-molecule protein sequencing technology sequences with single amino acid resolution in a real-time assay that does not require complex and expensive cyclical chemistry and fluidics.
With our technology, you benefit from:
- Deeper proteomic discoveries
- Easy protein sequencing workflow
- Affordable proteomic solutions
How Quantum-Si’s Technology Differs from Mass Spectrometry
Quantum-Si’s Platinum is designed to fit the space, budget, and existing expertise of most labs interested in generating insights from proteomics data.
Our technology uses unique kinetic binding signatures to identify proteins and detect PTMs, unlike mass spectrometry which is limited by mass:charge ratios and cannot distinguish protein variants with the same mass or PTMs.
Single-molecule sequencing from Quantum-Si offers you an affordable proteomics workflow that is easy to implement and delivers answers quickly without the need for extensive infrastructure or bioinformatics expertise.
How Quantum-Si’s Technology Differs from Immunoassays
Next Generation Protein Sequencing allows you to uncover exactly what is there and does not require specific antibodies for detection.
Quantum-Si’s technology does not require specialized target panels or additional NGS or PCR instrumentation to identify unique proteins. In addition, interrogation of PTMs and proteoforms is made possible with our sophisticated Cloud analysis software.
