Generate Deeper Insights With Protein Identification on Platinum™
With Next-generation Protein Sequencing™ at single-molecule resolution you can develop in-depth insights from protein identification studies.
Why Choose
Platinum?
Leveraging next-generation protein sequencing™ combined with intelligent workflow integration, Quantum-Si’s Platinum revolutionizes protein identification and elevates proteomics in your laboratory.
Convenient
Easily integrate protein identification into your existing research workflow with the benchtop Platinum protein sequencer and collaboration-friendly Cloud software.
Accessible
Every lab can quickly and accurately identify proteins with a simple workflow that automatically generates amino acid calls making data interpretation and discovery easy and accessible.
Insight-generating
With protein sequencing that’s sensitive and achieves single-molecule and single amino acid resolution, you can identify proteins without using affinity reagents, enabling unbiased discovery.
In this application note, we compare protein sequencing analysis of CDNF using Quantum-Si’s Platinum protein sequencing platform with mass spectrometry. The Platinum workflow offers an accessible and convenient solution for protein identification without the need for expensive equipment and expertise allowing researchers to gain deeper proteomic insights and make informed decisions about their protein samples.
Download the application note now to unlock a new level of understanding in your protein research.
While both mass spectrometry and the Quantum Si platform correctly identified the CDNF protein, the workflow was dramatically different.
Read the application note to see how the Platinum workflow can save you time and effort without compromising results.
Here we showcase CDNF peptide identification results from mass spectrometry and Platinum sequencing. Platinum sequencing resulted in unique identification of 6 peptides and demonstrates Platinum’s ability for accurate protein identification through a simplified workflow.
In this application note, we sequenced recombinant human CDNF (161 amino acids) as a model protein to demonstrate how to use the Platinum platform for protein identification. By combining sequencing data with proteome-wide mapping, we demonstrate identification of CDNF with extremely high confidence.
Learn about the workflow and review data by downloading the application note.
Only 4 recognizers are needed to identify 5 peptides in the CDNF protein. Since this data was published, a 5th recognizer capable of identifying amino acids, AST, has been added and further enhances confidence in identifying proteins such as CDNF.
Here we show an alignment of the sequence of one peptide fragment from CDNF to predicted sequencing traces from an in silico digestion of the entire human proteome.
Read the application note to see how many CDNF peptides were required for high-confidence protein identification.
Western blot, a prevalent method for protein identification, often faces challenges due to the need for quality antibodies and the inability to differentiate similar-sized proteins. This application note introduces a non-antibody-based approach, detailing an in-gel protein digestion and peptide extraction procedure. This innovative method integrates with Quantum-Si’s library preparation and protein sequencing workflows, allowing for the separation, enrichment, and identification of proteins using Quantum-Si’s Platinum next-generation protein sequencing™ instrument.
Curious about revolutionizing your protein identification process? Download the full application note to dive deeper!
Here we showcase an SDS-PAGE representation of CDNF Samples before the band excision process. The visual provides a clear indication of the bands that were selected for in-gel digestion and subsequent sequencing, marked by distinct orange and blue arrows. This serves as a foundational step in understanding the sample preparation process.
Figure 2 presents representative traces, coverage, and pulse duration data for peptides identified in the 10 µg sample of CDNF. With the use of five recognizers, the image illustrates the identification of 5 distinct peptides, offering a detailed insight into the sequencing results of the 10 µg CDNF sample.
This graph compares the relative number of peptide alignments for each in-gel digested sample and reveals that both the 5 µg and 10 µg samples produced a higher number of aligned CDNF peptides compared to the smaller samples. Additionally, the 10 µg Band B sample also yielded a significant count of CDNF-aligned peptides, providing valuable insights into the efficiency and results of the in-gel digestion process.
This application note introduces Quantum-Si’s Platinum instrument, which allows for the direct sequencing and identification of proteins in complex mixtures. By eliminating the need for targeted antibody-based assays, this technology offers a solution to detect multiple proteins simultaneously. We present a method for optimizing sample preparation and demonstrate the feasibility of identifying five distinct recombinant proteins in a mixture.
Download the full application note to learn more about this groundbreaking protein sequencing platform.
We tested the feasibility of detecting multiple proteins on Platinum by combining a mixture of five recombinant proteins (CDNF, FGF2, IL4, GMFB, and PDIA1) and employing protein sequencing to identify them.
To determine if overall peptide coverage would be affected by different library preparation methods, we compared two different mixes with increased complexity.
Read the application note for an in-depth discussion of our methods and results.
Quantum-Si introduces the groundbreaking Platinum system, a next-gen platform for single-molecule protein sequencing on a unique semiconductor chip. Integrated with the Quantum-Si Cloud, this system simplifies data analysis, offering a tailored experience for users to design experiments, automate workflows, and share findings securely. The Cloud platform adapts to individual research and workflow demands, transforming protein sequencing data into valuable insights.
Dive deeper into this revolutionary technology – download the application note now!
Discover the synergy between Platinum and the Cloud. This interface ensures efficient planning for sequencing runs. Once a sample is introduced into Platinum, the run commences, streaming data directly to the Cloud. This integration not only facilitates downstream data analysis but also provides real-time updates, ensuring that researchers are always in the loop.
Delve into the intricate data flow within the Quantum-Si Cloud. From accessing the platform to processing and analyzing data, every step is meticulously designed. Data remains encrypted, whether it’s being transferred, stored, or analyzed, prioritizing user data security. With role-based access and encrypted transfers, the Cloud guarantees the utmost protection for your research data.
Featured Resources
WHITE PAPER
Digitizing Protein Sequencing: Detecting Individual Amino Acids through Real-Time Photon Emission
PEER-REVIEWED ARTICLE
Real-Time Dynamic Single-Molecule Protein Sequencing on an Integrated Semiconductor Device
POSTER