Generate Deeper Insights with Protein Identification on Platinum® Pro
Deeply interrogate individual proteins with high resolution using next-gen protein sequencing.
Why choose next-gen protein sequencing (NGPS) for protein identification?
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Complete protein identification prior to additional experiments -
Identify unknown bands from your gels -
Determine which proteins are in a biological sample like serum -
Identify potential contaminants from protein production -
Verify that your antibody is successfully isolating the target protein
The ability to accurately identify proteins is essential to exploring function, understanding impact to biological process, and uncovering disease mechanisms.
NGPS with single-amino acid resolution offers the precision needed to avoid drawing inaccurate conclusions.
When analyzed with NGPS, your protein is digested into peptides and individually distributed across our sequencing chip. Each peptide is sequenced independently and generates distinct kinetic signatures that come from every single amino acid in your sequence.
These signatures are then mapped to a proteome-wide inference panel, enabling you to identify the proteins present in your sample. NGPS enables single-molecule level characterization of individual proteins from biological samples with precision and sensitivity.
Explore our resources to discover how Platinum Pro can enhance your protein identification.
Adeno-associated viruses (AAVs) are widely used as delivery vectors in clinical gene therapy, with each serotype exhibiting unique capsid protein sequences that influence tissue tropism, transduction efficiency, and immune response. Ensuring the identity and purity of AAV preparations is essential during manufacturing and quality control, particularly when closely related serotypes are involved. In this study, we demonstrate the use of NGPS to identify AAV8 and AAV9 capsid proteins by detecting three serotype-specific peptides capturing amino acid differences at position 105, 315, 539, and 540. NGPS enabled reliable identification of each serotype and unambiguous detection of each serotype from a mixture, with sensitivity sufficient to detect AAV8 and AAV9 at levels of 10% in a mixture based on distinct kinetic signatures. These results highlight the utility of NGPS as a high-resolution analytical tool for AAV serotype characterization and monitoring of contamination.
Sanavia Oncology is focused on developing antibody therapeutics targeting novel cancer markers, and plans to utilize NGPS to better characterize novel cancer targets, which is a crucial tool in their target identification workflow.
The vast complexity of the proteome currently overwhelms any single analytical technology in capturing the full spectrum of proteoform diversity. In this study, the team at Northwestern University evaluated the complementarity of two cutting-edge proteomic technologies—single-molecule protein sequencing and individual ion mass spectrometry—for analyzing recombinant human IL-6 (rhIL-6) at the amino acid, peptide, and intact proteoform levels. For single-molecule protein sequencing, they employed the Platinum® instrument. NGPS on Platinum utilizes cycles of N-terminal amino acid recognizer binding and aminopeptidase cleavage to enable parallelized sequencing of single peptide molecules. They found that NGPS produces single amino acid coverage of multiple key regions of IL-6, including two peptides within helices A and C which harbor residues that reportedly impact IL-6 function. For top-down proteoform evaluation, the team used individual ion mass spectrometry (I2MS), a highly parallelized orbitrap-based charge detection MS platform. Single ion detection of gas-phase fragmentation products (I2MS2) gives significant sequence coverage in key regions in IL-6, including two regions within helices B and D that are involved in IL-6 signaling. Together, these complementary technologies delivered a combined 52% sequence coverage, offering a more complete view of IL-6 structural and functional diversity than either technology alone.
Northwestern University’s Neil Kelleher and his team have a published article, featured in Springer’s Analytical and Bioanalytical Chemistry journal. The paper highlights the synergy of complementary protein detection methods to more comprehensively cover protein segments relevant to biological interactions.
Protein variants of the same gene–proteoforms–can have high molecular similarity yet exhibit different biological functions. Thus, identifying unique peptides that unambiguously map to proteoforms can provide crucial biological insights. In humans, four human tropomyosin (TPM) genes produce similar proteoforms that can be challenging to distinguish with standard proteomics tools.
Dr. Gloria Sheynkman and her team at the University of Virginia School of Medicine have a published article in the Journal of Proteome Research showcasing the capabilities of the Platinum NGPS in advancing their research in this complex area of study.
In this expert led webinar, Dr Danielle Tullman-Ercek, Professor of Chemical and Biological Engineering at the Northwestern University, and Katherine Johnson, Senior Director, Product, at Quantum-Si, Inc will discuss a novel NGPS platform, Platinum, and how it can complement immunoassays and other proteomic workflows by providing deeper insights into proteins. Learn how this NGPS technology works and what applications can be performed using this novel technology. Additionally, Dr. Tullman-Ercek will illustrate how protein identification on Platinum was utilized in her research to provide additional information about proteins typically studied via conventional western blot and mass spectrometry techniques. She will demonstrate how NGPS provides additional information about proteins, comparing results from conventional techniques to those obtained from NGPS.
Key learning objectives
- Learn about Next-Generation Protein Sequencing, how it works and key benefits
- Explore how it compares to and complements conventional proteomic techniques such as mass spectrometry and immunoassays
- Explore the applications that can be performed with Platinum next-generation sequencing technology
- Discover how Platinum is utilized to complement data obtained from conventional protein identification techniques to provide deeper insights into proteins
Speakers: Dr Danielle Tullman-Ercek, Professor of Chemical and Biological Engineering, Northwestern University and Katherine Johnson, Senior Director, Product, QSI Date: October 6, 2023
Sequencing proteins and correlating amino acid changes to biological function is critical to advancing our understanding of human health and disease. NGPS on Platinum enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument. To demonstrate the versatility of Platinum and the use of Kinetic Signatures, we sequenced various types of samples, including recombinant proteins, protein mixtures, immunoprecipitated proteins, peptide barcodes, and peptides with Post Translational Modifications (PTMs).
Furthermore, we showcased the detection of peptide barcodes along with the utilization of barcoding techniques to streamline protein engineering applications.
Recent product enhancements to the platform include new sequencing kit and chip chemistry as well as advanced analytical software tools. These enhancements unlock new applications such as the use of protein inference to study unknown protein samples.