Sequencing and Identification of Proteins from Five-protein Mixtures on Platinum®


Complex biological samples contain a diverse array of proteins, and understanding their presence and relative abundance is vital for unraveling the underlying biological processes related to human health and disease. However, traditional protein detection methods requiring antibodies or targeted reagents often face limitations in identifying proteins with variant peptide sequences. Quantum-Si’s Platinum® instrument provides an innovative solution by directly sequencing proteins, eliminating the need for antibody-based assays. In this application note, we demonstrate the successful identification of five recombinant proteins within a mixture with Platinum, showcasing the feasibility of detecting multiple proteins without the constraints of antibody specificity.