Posters Archives | Page 2 of 3

Next-Gen Protein Sequencing Resolves Peptide Variants Derived from Tropomyosin Proteoforms

Next-gen protein sequencing (NGPS) resolves proteotypic representing the three main types of regulation — genetic, splicing, and post-translational modifications — contributing to proteomic molecular diversity. Kinetic signatures produced by NGPS are a novel orthogonal datatype that can complement other commonly used proteomics approaches. NGPS data can be used for a range of protein characterization applications, including detecting […]

Single-molecule sequencing of immunopeptides and antigenic protein markers on the Platinum® instrument

Summary: Single-molecule protein sequencing offers the sensitivity needed to explore the immunopeptidome and profile tumor-associated antigens. We demonstrate the use of the Platinum benchtop instrument to sequence immunopeptides and proteins at the single-molecule level. Peptides are loaded into nanoscale chambers on a chip, where dye-labeled N-terminal amino acid recognizers bind and generate distinct kinetic patterns. […]

Beyond the Genome: Unraveling Protein Variability with Quantum-Si’s Next-Gen Protein Sequencing Technology

Summary: Protein sequencing is a groundbreaking advancement in proteomics that augments genomics and transcriptomics research by providing crucial insights into the functional proteins encoded by the genome. Protein sequencing offers a more complete understanding of cellular processes and disease mechanisms by detecting changes at the protein level, such as post-translational modifications (PTMs), which cannot be […]

Integrated sequencing of transcripts and proteins at the single molecule level to detect isoforms and proteotypic peptides in filament biomarkers

Summary: In collaboration with the University of Virginia, this poster describes the integration of long-read RNA sequencing of transcript isoform with next-generation protein sequencing via Platinum® to characterize related tropomyosin (TPM1/2) proteoforms. As paralogous and alternatively spliced proteoforms are important in human health and disease, single molecule sequencing with orthogonal methods facilitates proteogenomics and offers […]

Next-Gen Protein Sequencing integrates with top-down MS to distinguish native and artifactual proteoforms

Summary: In collaboration with Northwestern University, we utilized next-generation protein sequencing (NGPS) via Platinum® and top-down mass spectrometry (TD-MS) to discern peptides with N-terminal glutamine (Q) versus pyroglutamate (pE) – a post-translational modification (PTM) important for bioactivity and implicated in neurodegeneration. As pE is not only generated via biological processes but also common sample prep/instrument […]

Beyond the Genome: Identification of Protein Sequence and PTMs with Quantum-Si’s Next-Gen Protein Sequencer Platinum®

Summary: To demonstrate the versatility of Platinum and the use of kinetic signatures, we sequenced various types of samples, including mixtures of recombinant proteins, peptides with post-translational modifications (PTMs), proteins immunoprecipitated from human serum, and proteins isolated from human serum via fractionation with SDS-PAGE. The resulting peptides generated distinct kinetic signatures aligned to their respective […]

Advancing Cancer Research with Next-Gen Protein Sequencing on Platinum® by Quantum-Si

Summary: Sequencing proteins and correlating amino acid changes to biological function is critical to advancing cancer research. Next-generation protein sequencing (NGPS) on Platinum enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument.

Enzymatic Addition of Azides to C-termini for Single-Molecule Sequencing

Summary: The enzymatic addition of C-terminal azides to polypeptides (ENZ-A-CAP for short) aims to expand on Quantum-Si’s Platinum® single-molecule sequencing platform to enable protein sequencing of peptides generated with any digest patterns and hopes to extend this feature to full-length proteins for full proteoform sequencing.

Quantum-Si’s Next-Gen Protein Sequencer Enables Protein Detection and Peptide Characterization

Summary: Sequencing proteins and correlating amino acid changes to biological function is critical to advancing our understanding of human health and disease. Next-generation protein sequencing (NGPS) on Platinum® enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument. To demonstrate the versatility of Platinum and the […]

Measuring Nanobody Kinetics at the Single-Molecule Level

Summary: Antibodies are a paradigm for high-affinity, protein-based binding reagents and are extremely important in biotechnological, diagnostic, and therapeutic applications. Of special interest are nanobodies, recombinant variable domains from heavy-chain-only antibodies. Nanobodies have several advantages: their small molecular weight, superior solubility and stability and clearance rate. One particular use of nanobodies is their use in […]