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Next-Generation Protein Sequencing Resolves Peptide Variants Derived from Tropomyosin Proteoforms

US HUPO 2025 Poster – next-generation protein sequencing (NGPS) resolves proteotypic representing the three main types of regulation — genetic, splicing, and post-translational modifications — contributing to proteomic molecular diversity. Kinetic signatures produced by NGPS are a novel orthogonal datatype that can complement other commonly used proteomics approaches. NGPS data can be used for a range of […]

Beyond the Genome: Advancing our Understanding of the Proteome with Next-Gen Protein Sequencing

Protein information is a crucial component of multiomics workflows, offering insights that integrate genetic, transcriptomic, and proteomic data for a more comprehensive understanding of biological systems. Multiomics studies would greatly benefit from methods that enable direct protein detection and characterization with exceptional sensitivity. In this context, we present the unique capabilities of next-generation protein sequencing […]

Quantum-Si’s Next-Gen Protein Sequencer Enables Protein Detection and Peptide Characterization from Biological Samples

Studies of the proteome would greatly benefit from methods that enable direct protein detection and peptide characterization with exceptional sensitivity. In this context, we present the unique capabilities of next-gen protein sequencing on Quantum-Si’s Platinum® instrument, which utilizes single-molecule protein sequencing to achieve detection of unknown proteins and protein variants. Individual peptides are digested from […]

University of Virginia logo with an orange Rotunda graphic on the left and University of Virginia in blue text on the right.

Next-Gen Protein Sequencing Resolves Peptide Variants Derived from Tropomyosin Proteoforms

Next-gen protein sequencing (NGPS) resolves proteotypic representing the three main types of regulation — genetic, splicing, and post-translational modifications — contributing to proteomic molecular diversity. Kinetic signatures produced by NGPS are a novel orthogonal datatype that can complement other commonly used proteomics approaches. NGPS data can be used for a range of protein characterization applications, including detecting […]

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Single-molecule sequencing of immunopeptides and antigenic protein markers on the Platinum® instrument

Summary:  Single-molecule protein sequencing offers the sensitivity needed to explore the immunopeptidome and profile tumor-associated antigens. We demonstrate the use of the Platinum benchtop instrument to sequence immunopeptides and proteins at the single-molecule level. Peptides are loaded into nanoscale chambers on a chip, where dye-labeled N-terminal amino acid recognizers bind and generate distinct kinetic patterns. […]

Beyond the Genome: Unraveling Protein Variability with Quantum-Si’s Next-Gen Protein Sequencing Technology

Summary:  Protein sequencing is a groundbreaking advancement in proteomics that augments genomics and transcriptomics research by providing crucial insights into the functional proteins encoded by the genome. Protein sequencing offers a more complete understanding of cellular processes and disease mechanisms by detecting changes at the protein level, such as post-translational modifications (PTMs), which cannot be […]

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Integrated sequencing of transcripts and proteins at the single molecule level to detect isoforms and proteotypic peptides in filament biomarkers

Summary:  In collaboration with the University of Virginia, this poster describes the integration of long-read RNA sequencing of transcript isoform with next-generation protein sequencing via Platinum® to characterize related tropomyosin (TPM1/2) proteoforms. As paralogous and alternatively spliced proteoforms are important in human health and disease, single molecule sequencing with orthogonal methods facilitates proteogenomics and offers […]

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Next-Gen Protein Sequencing integrates with top-down MS to distinguish native and artifactual proteoforms

Summary:  In collaboration with Northwestern University, we utilized next-generation protein sequencing (NGPS) via Platinum® and top-down mass spectrometry (TD-MS) to discern peptides with N-terminal glutamine (Q) versus pyroglutamate (pE) – a post-translational modification (PTM) important for bioactivity and implicated in neurodegeneration. As pE is not only generated via biological processes but also common sample prep/instrument […]

Beyond the Genome: Identification of Protein Sequence and PTMs with Quantum-Si’s Next-Gen Protein Sequencer Platinum®

Summary:  To demonstrate the versatility of Platinum and the use of kinetic signatures, we sequenced various types of samples, including mixtures of recombinant proteins, peptides with post-translational modifications (PTMs), proteins immunoprecipitated from human serum, and proteins isolated from human serum via fractionation with SDS-PAGE. The resulting peptides generated distinct kinetic signatures aligned to their respective […]

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