Integrated sequencing of transcripts and proteins at the single molecule level to detect isoforms and proteotypic peptides in filament biomarkers

Summary: 

In collaboration with the University of Virginia, this poster describes the integration of long-read RNA sequencing of transcript isoform with Next-Generation Protein Sequencing™ via Platinum® to characterize related tropomyosin (TPM1/2) proteoforms. As paralogous and alternatively spliced proteoforms are important in human health and disease, single molecule sequencing with orthogonal methods facilitates proteogenomics and offers new avenues for biomedical applications.