Integrated sequencing of transcripts and proteins at the single molecule level to detect isoforms and proteotypic peptides in filament biomarkers


In collaboration with the University of Virginia, this poster describes the integration of long-read RNA sequencing of transcript isoform with Next-Generation Protein Sequencing™ via Platinum® to characterize related tropomyosin (TPM1/2) proteoforms. As paralogous and alternatively spliced proteoforms are important in human health and disease, single molecule sequencing with orthogonal methods facilitates proteogenomics and offers new avenues for biomedical applications.