Immunoprecipitation of IL-6 from Human Serum for Next-Gen Protein Sequencing on Platinum®

The enrichment of low-abundant proteins from complex biological matrices is a key step for many proteomic workflows. In this technical note, we present an immunoprecipitation protocol compatible with Quantum-Si’s next-gen protein sequencing workflow. This process utilizes an on-bead digestion protocol that forgoes the sample elution step and proceeds directly to the preparation of digested peptide […]

Comparison of Protein Sequencing Analysis of CDNF, IL6, and FGF2 on Platinum® and Mass Spectrometry

Mass Spectrometry has been the gold standard for deciphering information about a protein’s amino acid sequence.  However, mass spectrometry is not convenient and accessible to everyday scientists with its large capital equipment expense and advanced expertise requirement. Scientists often need to resort to using core facilities to sequence proteins. Furthermore, mass spectrometry infers amino acids […]