Immunoprecipitation of IL-6 from Human Serum for Next-Gen Protein Sequencing on Platinum®
The enrichment of low-abundant proteins from complex biological matrices is a key step for many proteomic workflows. In this technical note, we present an immunoprecipitation protocol compatible with Quantum-Si’s next-gen protein sequencing workflow. This process utilizes an on-bead digestion protocol that forgoes the sample elution step and proceeds directly to the preparation of digested peptide […]