human serum Archives

Achieving Nanogram (Femtomole) Detection Limits of Human Serum Albumin from In-Gel Digestion Coupled with Next-Generation Protein Sequencing on Platinum® Pro

The limit of detection (LOD) in protein sequencing is essential for accurate and comprehensive proteome analysis, especially for identifying low-abundance proteins in biological samples. To evaluate the LOD of the Platinum Pro next-gen protein sequencing instrument, varying amounts of human serum albumin (ranging from 1 ng to 30 μg) were analyzed using SDS-PAGE. Protein bands […]

Immunoprecipitation of IL-6 from Human Serum for Next-Gen Protein Sequencing on Platinum®

The enrichment of low-abundant proteins from complex biological matrices is a key step for many proteomic workflows. In this technical note, we present an immunoprecipitation protocol compatible with Quantum-Si’s next-gen protein sequencing workflow. This process utilizes an on-bead digestion protocol that forgoes the sample elution step and proceeds directly to the preparation of digested peptide […]