Achieving Nanogram (Femtomole) Detection Limits of Human Serum Albumin from In-Gel Digestion Coupled with Next-Generation Protein Sequencing on Platinum® Pro 

The limit of detection (LOD) in protein sequencing is essential for accurate and comprehensive proteome analysis, especially for identifying low-abundance proteins in biological samples. To evaluate the LOD of the Platinum Pro next-gen protein sequencing instrument, varying amounts of human serum albumin (ranging from 1 ng to 30 μg) were analyzed using SDS-PAGE. Protein bands […]