Western blot is one of the most widely used methods for protein identification but achieving reliable and reproducible results depends on quality antibodies with validated specificity for the target protein. Furthermore, differences in amino acid sequences and isoforms cannot be easily differentiated by western blot if the size of the protein is similar. To overcome […]
Nucleic acid-based therapeutics, including antisense oligonucleotides (ASO), small interfering RNA (siRNA), messenger RNA (mRNA), immunomodulatory DNA/RNA, and gene-editing guide RNA (gRNA), are promising because they can treat diseases by targeting their genetic information directly in vivo. These treatments can provide long-lasting or even curative effects by inhibiting, adding, replacing, or editing genes. This technical note […]
Protein analysis is crucial in cancer research because proteins play pivotal roles in cancer development, progression, and response to treatment, serving as potential biomarkers and therapeutic targets. Cutting-edge techniques like next-gen protein sequencing enable scientists to unravel the complexities of altered proteins and their interactions, advancing our understanding of cancer detection, prognosis, and novel therapeutic strategies. […]
The limit of detection (LOD) in protein sequencing is essential for accurate and comprehensive proteome analysis, especially for identifying low-abundance proteins in biological samples. To evaluate the LOD of the Platinum Pro next-gen protein sequencing instrument, varying amounts of human serum albumin (ranging from 1 ng to 30 μg) were analyzed using SDS-PAGE. Protein bands […]
Next-gen protein sequencing (NGPS) on Platinum enables protein identification and characterization with single-molecule and single amino acid resolution in a simple workflow using a benchtop instrument. Platinum Analysis Software workflows make peptide alignment and protein mapping easy to interpret without the need for advanced expertise. The output files from Platinum Analysis Software provide researchers with […]