Single-Molecule Protein Sequencing on the Quantum-Si Platform: Advances in Protein and Proteoform Identification and Comparison With Mass Spectrometry

Summary:

Studies of the proteome would benefit greatly from methods to directly sequence and digitally quantify proteins and detect post-translational modifications with single-molecule sensitivity. Quantum-Si™ has developed the Platinum™ next-generation protein sequencing platform to offer a convenient benchtop solution for direct sequencing of single protein molecules with the aim of making protein sequencing accessible to any research lab. Quantum-Si has improved fundamentals of the assay components and platform allowing for increased coverage across the proteome. This poster describes these Platinum improvements and how they have resulted in unprecedented read depth and the identification of previously unseen proteins, leading to new insights and greater confidence in protein identification.