Reducing Protein Input for Next-Generation Protein Sequencing™ on the Platinum® Instrument

US HUPO 2025 Poster – Next-Generation Protein Sequencing™ (NGPS™) on the Quantum-Si Platinum® and Platinum® Pro instruments enable the direct analysis of individual proteins at an unprecedented resolution.¹ However, one challenge in applying this technology to biological and clinical samples is the limited availability of input protein. Quantum-Si’s Library Preparation Kit, V1 recommends a protein input of 500 pmol for library preparation, which often means enrichment is required when working with clinical and biological samples. Quantum-Si recently developed an updated version (V2) of the Library Prep Kit and protocol to address this limitation, introducing new chemistry to improve conjugation efficiency and reduce protein input requirements.

^{1 Brian D. Reed et al, Science 2022, 378 (6166) 186–192.}