Streamline Protein Screening
Protein Barcoding with Next-Gen Protein Sequencing
Next-gen protein sequencing (NGPS) offers single-molecule sequencing of multiplexed protein barcodes with quantitative results.
Drug Delivery
Evaluate and optimize drug delivery vehicles like LNPs or AAVs with protein barcodes
Protein Engineering
Efficiently analyze protein characteristics in multiplex with protein barcodes
Multiplexed Protein Screening Solution
Direct Protein Detection
Quantify expression and differentiate
functional performance of proteins
Multiplexing Power
Test multiple candidates in a single-model organism with up to 24× the power of single-plex analysis
Analytical Validity
Detect low-abundance proteins and differentiate expression changes across up to 240-fold dynamic range for a full chip and 120-fold dynamic range for a half chip
Workflow Optimization
Total barcode library workflow time <6 hours with 1 hour hands-on time
Advance Drug Delivery and Protein Analysis with Precision Barcoding
Characterize multiplexed drug delivery and translation efficiency of mRNA therapeutics with lipid nanoparticles (LNPs). Multiplexed analysis of target therapeutics and drug delivery systems accelerates
screening cycles, reduces resources of in vivo and in vitro models, and saves time.
Barcode mix encoded as mRNA and packaged into LNPs
Inject into mouse model; harvest target tissues
Enrich for target protein; functionalize, cleave, and sequence barcodes
How it Works
Protein barcoding provides users with a robust method to screen multiple protein variants in a single pooled reaction. Individual proteins are tagged with short, unique peptide barcodes that identify each variant. With the barcoding kit, screened proteins are prepared and barcodes are released for sequencing on Platinum® Pro.
Prepare peptide barcodes for single-molecule sequencing
Sequence peptide barcodes with single-molecule precision
Analyze multiplexed barcodes with relative quantitation
Protein Sequencing Kits
Achieve deeper protein insights with ready-to-sequence chips, recognizers, and aminopeptidases.
Library Preparation Kits
High-confidence protein sequencing from nanogram inputs with the Library Prep Kit.
Barcoding Kits
Characterize multiple protein variants simultaneously using protein barcodes on Platinum Pro.
Explore these resources to discover how our barcoding solutions can enhance your protein research
Protein barcoding has emerged as a transformative tool for the multiplexed identification and characterization of proteins, providing a mechanism for precise tracking of protein affinity, location, and expression. In this study, we describe the development of a protein barcoding workflow for use with single-molecule Next-Generation Protein Sequencing (NGPS) on the benchtop Platinum instrument.
This paper presents data on the validation of eight peptide barcodes, each designed to minimize detection bias and maximize sensitivity across various experimental conditions.
The Platinum instrument, kits, and software contain everything you need to prepare, sequence, and analyze proteins. The Barcoding Kit is a new initial step in Quantum-Si’s Next-Generation Protein Sequencing workflow. The Barcoding Kit prepares and immobilizes barcode-tagged peptides for sequencing.
The Platinum instrument fits on your benchtop and seamlessly integrates into your existing workflow, enabling protein sequencing right after sample preparation and chip loading.
Equipped with a user-friendly interface, Platinum allows you to easily start your protein sequencing run following a few prompts. Sequencing data is generated and can be automatically transferred to the Platinum Analysis Software for analysis.
This webpage explains how protein barcoding — combined with Quantum-Si’s Next-Generation Protein Sequencing (NGPS) solution — offers a transformative solution by enabling multiplexed protein variant screening and characterization with significantly faster assay speeds and enhanced throughput.
Explore this case study to learn how researchers are using the protein barcoding application on Platinum® to accelerate development of novel gene therapies with improved targeting capabilities. With protein barcoding you can:
- Accelerate the screening and optimization of lipid nanoparticle (LNP) delivery vehicles for targeted gene therapies.
- Precisely track LNP delivery to specific tissues, enhancing precision and efficiency.
- Utilize a cost-effective method as an accessible alternative to mass spectrometry for in vivo delivery studies.
Explore the case study to understand how combining next-generation protein sequencing with protein barcoding facilitates high-throughput mapping of protein conformations and phenotypic effects, without the limitations of traditional methods. With protein barcoding, you can:
- Conveniently screen hundreds of variants using protein barcoding in your protein expression system
- Utilize the diversity of protein barcodes to screen proteins with diverse characteristics
- Expand your research and uncover innovative strategies for preventing and treating various diseases