Next-gen protein sequencing (NGPS) resolves proteotypic representing the three main types of regulation — genetic, splicing, and post-translational modifications — contributing to proteomic molecular diversity. Kinetic signatures produced by NGPS are a novel orthogonal datatype that can complement other commonly used proteomics approaches. NGPS data can be used for a range of protein characterization applications, including detecting tissue-specific variant expression, epitope mapping, and PTM profiling.

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