High-resolution detection of post-translational modifications using single-molecule protein sequencing

ASMS 2026 Poster – Post-translational modifications (PTMs) are critical regulators of protein function  and are extremely diverse in both types and frequencies¹

While many PTMs can be readily identified, identification and site localization can still be challenging when the PTM introduces small mass changes or when multiple modification sites are possible

Here, we demonstrate how N-terminal amino acid (NAA) recognizer-based sequencing, an emerging single-molecule protein sequencing technology, can detect a variety of PTMs based on changes in recognizer binding kinetics

¹ Aebersold, R. et al. How many human proteoforms are there? Nat. Chem. Biol. 14, 206-214 (2018).