Case Study

Use of Protein Barcodes and Next-Generation Protein Sequencing™ for High-Throughput Mapping of Protein Conformations & Their Phenotypic Effects

Eugene Serebryany Ph.D.
Assistant Professor, Department of Physiology and Biophysics
Stony Brook University

“There’s a vast conformational space of proteins that remains to be explored due to a lack of high-throughput experimental methods. We’re advancing a novel technique that combines protein barcodes and next generation, single-molecule protein sequencing to enable systematic, high-throughput mapping of non-native conformations that can’t be crystallized, and then link them to phenotypic effects. The combination of single-molecule sequencing and protein barcodes has the potential to dramatically expand this field of study.”

Explore the case study to understand how combining next-generation protein sequencing with protein barcoding facilitates high-throughput mapping of protein conformations and phenotypic effects, without the limitations of traditional methods.