Protein Identification via Next-Generation Protein Sequencing and Proteome-Wide Mapping
Summary
Protein identification is of fundamental importance in many areas of proteomics. Applications include determination of the presence or absence of an expected protein in a sample of interest, identification of an unknown protein present in a biological sample, and identification of a protein responsible for a biochemical activity in an isolated protein fraction. In some cases, mass spectrometry or affinity-based methods may be suitable options, but these methods can face substantial challenges when the protein of interest is unknown, with certain amino acid sequences or reliable detection of post-translational modifications (PTMs). To offer a solution based on direct sequencing and to provide more accessible tools for discovery in protein science, Quantum-Si has integrated our single-molecule sequencing output with automated cloud-based algorithms for proteome-wide mapping of sequencing data to enable accurate protein identification.