Resource Category: App Notes

Summary: The enrichment of low-abundant proteins from complex biological matrices is a key step for many proteomic workflows. In this technical note, we present an immunoprecipitation protocol compatible with Quantum-Si’s next-generation protein sequencing™ workflow. This process utilizes an on-bead digestion protocol that forgoes the sample elution step and proceeds directly to the preparation of digested…

Summary: Mass Spectrometry has been the gold standard for deciphering information about a protein’s amino acid sequence.  However, mass spectrometry is not convenient and accessible to everyday scientists with its large capital equipment expense and advanced expertise requirement. Scientists often need to resort to using core facilities to sequence proteins. Furthermore, mass spectrometry infers amino…

Summary: Identifying low-abundance monoclonal antibodies in a population of polyclonal antibodies can provide critical insights for diverse applications such as understanding disease mechanisms, disease diagnostics, vaccine development, therapeutic antibody discovery, and serological surveillance. Existing methodologies, despite their utility, come with significant disadvantages including high costs, complex procedures, and time-intensive workflows, which often limit their broad…

Summary:Proteins are the main structural and functional components of biological cells. Assembled and structured through the complexfolding of a specific sequence of amino acids, proteins, along with their dynamic regulation and post-translational modifications (PTMs), largely determine cellular phenotype. New tools to study these changes are necessary. Quantum-Si’s technology uses a proprietary, novel sequencing approach– […]

Summary: The identification and analysis of protein markers in biofluids play a crucial role in disease diagnosis. Urine, as a non-invasive sample source, enables the monitoring of disease progression through biomarker analysis. However, traditional methods for detecting biomarkers often overlook important protein variants and require complex techniques. The Quantum-Si’s next-generation protein sequencing™ Platinum™ workflow has…

Summary: Complex biological samples contain a diverse array of proteins, and understanding their presence and relative abundance is vital for unraveling the underlying biological processes related to human health and disease. However, traditional protein detection methods requiring antibodies or targeted reagents often face limitations in identifying proteins with variant peptide sequences. Quantum-Si’s Platinum™ instrument provides…

Summary: Accurate and rapid identification of SARS-CoV-2 variants is crucial for effective surveillance and disease monitoring. Furthermore, patients with long COVID may still have residual spike protein in their blood. Distinguishing variants in long COVID patients via protein sequencing could lead to new understandings and treatment of the disease. In this application note, we present a…

Summary:Proteins are the main structural and functional components of biological cells. Assembled and structured through the complexfolding of a specific sequence of amino acids, proteins, along with their dynamic regulation and post-translational modifications (PTMs), largely determine cellular phenotype. New tools to study these changes are necessary. Quantum-Si’s technology uses a proprietary, novel sequencing approach– […]

Summary:Proteins are the main structural and functional components of biological cells. Assembled and structured through the complexfolding of a specific sequence of amino acids, proteins, along with their dynamic regulation and post-translational modifications (PTMs), largely determine cellular phenotype. New tools to study these changes are necessary. Quantum-Si’s technology uses a proprietary, novel sequencing approach– […]